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Fractionation of polyclonal antibody by isoelectric focusing and chromatofocusing: Separation of high-affinity rabbit clonotype anti-thyroxine antibody

✍ Scribed by Yuichi Endo; Kiyoshi Miyai; Naoshige Hata; Kiyoshi Ichihara


Book ID
107712124
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
551 KB
Volume
144
Category
Article
ISSN
0003-2697

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Fractionation of polyclonal antibody by
✍ Yuichi Endo; Kiyoshi Miyai; Naoshige Hata; Kiyoshi Ichihara πŸ“‚ Article πŸ“… 1984 πŸ› Elsevier Science 🌐 English βš– 734 KB

Immunoglobulin G (IgG) fractions prepared from three different batches of rabbit antihuman thyrotropin (hTSH) antisera were fractionated by agarose isoelectric focusing (IEF) in the pH ranges 3 to 10 and 5 to 8. Staining of protein in agarose gel after IEF showed that polyclonal IgG separated into m