Fractionation of calf thymus histone fractions 2a and 3 on sephadex
β Scribed by Lubomir S. Hnilica; Lloyd G. Bess
- Publisher
- Elsevier Science
- Year
- 1964
- Tongue
- English
- Weight
- 276 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Amberlite IR-120, a polystyrene sulphonate type of cation exchanger, equilibrated with A1 3+ ions, has been employed for the fractionation of whole histone. This adsorbent permits the quantitative and reproducible recovery of whole histone in six fractions.
## Abstract This laboratory recently reported the development of a biotinβcellulose/streptavidin affinity chromatography method based on the DNase I sensitivity of active chromatin to isolate a DNA fraction from murine erythroleukemia (MEL) cells that is more than 15βfold enriched in active genes (
## Abstract De novo DNA methyltransferases, Dnmt3a and 3b, were purified by fractionation of Sβ100 extract from mouse lymphosarcoma cells through several chromatographic matrices followed by glycerol density gradient centrifugation. Dnmt3a was separated from Dnmt3b and Dnmt1 in the first column, Qβ