Fractionation and characterisation ofj water-soluble polysaccharide-protein complexes containing hydroxyproline from the leaves of Vida faba
✍ Scribed by A. Pusztai; R. Begbie; I. Duncan
- Publisher
- John Wiley and Sons
- Year
- 1971
- Tongue
- English
- Weight
- 524 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0022-5142
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✦ Synopsis
The water-soluble polysaccharide-protein complexes obtained previously by extraction with aqueous tvichloroacetic acid and by phenol-aqueous (pH 8) partitioning fvom the leaves of Vicia faba were fractionated into a number of components by continuous high voltage electrophoresis at pH 8 . 3 and 3.97 and by Sepharose 4B chromatography. The results indicated that these materials contained a spectrum of molecules with rather similar properties. All fvactions obtained contained about 4-7 ( w l w ) tightly bound protein, two-thirds of which was made up of four amino acids: hydroxyproline, alanine, serine and threonine. Similarly, all materials contained uronic acid (10-70 %; w / w ) and neutral sugars (I0-90%; w/w), mainly arabinose and galactose. High iironic acid content was found to be associated with appreciable rhamnose content and an arabinose-galactose ratio close to 2. This ratio became about 0.3 when the amount of uronic acids decreased to about 10% (w/w).