𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Four gel systems for electrophoretic fractionation of membrane proteins using ionic detergents

✍ Scribed by Fairbanks, Grant ;Avruch, Joseph


Book ID
102438342
Publisher
Wiley (John Wiley & Sons)
Year
1972
Tongue
English
Weight
435 KB
Volume
1
Category
Article
ISSN
0091-7419

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✦ Synopsis


Membrane p r o t e i n s were f r a c t i o n a t e d e l e c t r o p h o r e t i c a l l y i n p o l y a c r y l a m i d e g e l s c o n t a i n i n g e i t h e r a n i o n i c o r c a t i o n i c d e t e r g e n t a t e i t h e r pH 2.4 o r 8.3. I n a l l f o u r systems, p o l y p e p t i d e s m i g r a t e d as monomers b e a r i n g t h e charge o f t h e d e t e r g e n t i o n and s e m i -l o g a r i t h m i c m o l e c u l a r w e i g h t --m o b i l i t y r e l a t i o ns h i p s p e r t a i n e d . E l e c t r o p h o r e s i s o f e r y t h r o c y t e membrane p r o t e i n s i n t h e s e systems y i e l d e d v e r y s i m i l a r p r o t e i n s t a i n i n g p a t t e r n s b u t r e v e a l e d s i g n i f i c a n t d i f f e r e n c e s i n s i a l o g l y c o p r o t e i n m i g r a t i o n . The s t u d y o f membrane p r o t e i n s has been g i v e n g r e a t impetus by t h e development o f methods f o r e l e c t r o p h o r e t i c f r a c t i o n a t i o n o f p o l y p e p t i d e s i n p o l y a c r y l a m i d e g e l s c o n t a i n i n g SDS' ( 1 -4 ) .

been d i r e c t e d a t e l u c i d a t i n g t h e b a s i s o f t h e f r a c t i o n a t i o n , i n t r o d u c i n g Numerous i n v e s t i g a t i o n s have


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