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Four-color flow cytometric analysis of peripheral blood donor cell chimerism

✍ Scribed by Diana Metes; Alison Logar; William A Rudert; Adriana Zeevi; Jennifer Woodward; Anthony J Demetris; Kareem Abu-Elmagd; Bijan Eghtesad; Ron Shapiro; John J Fung; Massimo Trucco; Thomas E Starzl; Noriko Murase


Book ID
117415231
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
679 KB
Volume
64
Category
Article
ISSN
0198-8859

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Background: Human peripheral blood lymphocytes kept in culture after isolation die by an apoptotic process. Detection of apoptosis with labeled Annexin V to demonstrate loss of plasma membrane asymmetry is sensitive, specific, and easy using flow cytometry. This is true in lymphoblastic cell lines w