Forward-scattering degenerate four-wave mixing as a potential laser-based absorption detection method in liquid separation systems: Coupling to conventional-size liquid chromatography
✍ Scribed by T. de Beer; G.Ph. Hoornweg; G.J. Grootendorst; N.H. Velthorst; C. Gooijer
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 849 KB
- Volume
- 330
- Category
- Article
- ISSN
- 0003-2670
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✦ Synopsis
An explorative study on the compatibility of liquid separation systems, such as (micro) liquid chromatography (LC) and capillary electrophoresis (CE), and forward-scattering degenerate four-wave mixing (F-D4WM) as a detection method is presented. F-D4WM is a laser-based technique showing some analogy with holographic spectroscopy: a signal on a theoretical dark background is observed as a result of light absorption by an analyte. Parameters considered are solvent composition lfocussing on acetonitrile, methanol and water; mobile phases in LC and CE), detector cell construction, and influences of laser beam powers. A specially designed detector cell has been developed to meet the Brewster condition, both at the airquartz and the quartz-liquid boundaries. For practical reasons, the tested cell has an optical pathlength of 1 mm; reduction to lOOurn is required to apply the cell in microseparations. The F-D4WM technique has been involved for detection in a conventional-size, reversed-phase LC separation of l-and 2aminoantbraquinones. The detection limit obtained (for the 1 mm cell) is 2x 10e5 absorbance units. The experiments indicate that further reduction of background deserves explicit attention.