Using rat skin, we studied the influence of fat cells on the proliferation and differentiation of organoid hair follicle cells in a three-dimensional collagen gel matrix culture system. We cultured organoid hair follicles embedded in collagen gel under each of the following three conditions: cell-fr
✦ LIBER ✦
Formation of spheroids composed of Henle layer cells and fat droplet in the cultures of rat hair follicles
✍ Scribed by Justyna Niderla-Bielińska; Ewa Jankowska-Steifer; Stanisław Moskalewski
- Publisher
- Springer-Verlag
- Year
- 2006
- Tongue
- English
- Weight
- 355 KB
- Volume
- 298
- Category
- Article
- ISSN
- 0340-3696
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## Abstract Colonies of small hepatocytes appeared after the culture of primary adult rat hepatocytes for 4 days in serum‐free Dulbecco's modified Eagle's medium containing 10 mM nicotinamide and 10 ng/ml of epidermal growth factor (EGF), acidic and basic fibroblast growth factors (FGF), hepatocyte