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Formation of reactive oxygen species and of 8-hydroxydeoxyguanosine in DNA in vitro with betel quid ingredients

✍ Scribed by Urmila J. Nair; Robert A. Floyd; Jagadeesan Nair; Valeria Bussachini; Marlin Friesen; Helmut Bartsch


Book ID
113137056
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
653 KB
Volume
63
Category
Article
ISSN
0009-2797

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✍ Jia-Ling Yang; Lih-Chiann Wang; Chun-Yao Chang; Tsung-Yun Liu πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 English βš– 150 KB

To investigate DNA damage induced by Pb2+ and its prevention by scavengers, we determined DNA strand breakage and the formation of 8-hydroxydeoxyguanosine (8-OHdG) in DNA using plasmid relaxation assay and HPLC with electrochemical detection, respectively. Lead acetate induced DNA strand breakage in