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Formation of plasmid F' recombinants in recombination-deficient Escherichia coli cells and their properties

✍ Scribed by A. P. Pekhov; S. N. Rudneva

Publisher
Springer US
Year
1977
Tongue
English
Weight
299 KB
Volume
84
Category
Article
ISSN
0007-4888

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The F' plasmids ORF-1 (purE+ tsxs proC+ lac+) and F'14 (argE+ metB+ ilv+) contain active regions of recombination, fre I and fre II correspondingly. The plasmid ORF-1 is stable in recF- cells (i.e., with the RecBC pathway of recombination) and decays in rec+ cells (RecBCF pathway) giving two types o