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Formation of molten globule-like state during acid denaturation of Aspergillus niger glucoamylase

✍ Scribed by Mohammed Suleiman Zaroog; Saad Tayyab

Book ID
113863015
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
654 KB
Volume
47
Category
Article
ISSN
1873-3298

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## Abstract Titration of a salt‐free solution of native staphylococcal nuclease by HC1 leads to an unfolding transition in the vicinity of pH 4, as determined by near‐ and far‐UV circular dichroism. At pH 2‐3, the protein is substantially unfolded. The addition of further HC1 results in a second tr