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Formation of methylmalonyl-CoA and succinyl-CoA by extracts of Mycobacterium smegmatis

✍ Scribed by Rune L. Stjernholm; Rudolf E. Noble; Dieter Koch-Weser

Book ID
115791240
Publisher
Elsevier Science
Year
1962
Weight
255 KB
Volume
64
Category
Article
ISSN
0006-3002

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We developed gas chromatography-mass spectrometry assays for the concentration and mass isotopomer distribution of propionyl-CoA, methylmalonyl-CoA, and succinyl-CoA in tissues. The assays involves perchloric acid extraction of the tissue, spiking the extract with [ 2 H 5 ]propionyl-CoA and [ 2 H 4