The endocytosis of 125I-labeled asialofetuin by rat hepatocytes was studied using Nycodenz/sucrose gradients. It was shown in pulse chase experiments that the ligand endocytosed initially (after 1/2 to 1 min) was in small, slow-sedimenting vesicles of similar sizes. The vesicles containing the ligan
Formation of isotonic Nycodenz gradients for cell separations
โ Scribed by T.C. Ford; D. Rickwood
- Book ID
- 102627419
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- English
- Weight
- 423 KB
- Volume
- 124
- Category
- Article
- ISSN
- 0003-2697
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โฆ Synopsis
Nycodenz. is a new nonionic iodinated gradient medium which readily dissolves in water to give nontoxic, autoclavable solutions. This paper describes the use of diffusion techniques to prepare isotonic Nycodenz gradients with maximum densities up to 1.15 g/ml, which is sufficient to band most types of cells.
๐ SIMILAR VOLUMES
Nycodenz is a new nonionic iodinated density gradient medium which has several advantages over metrizamide. Although, overall. biological samples band at similar densities in Nycodenz and metrizamide gradients, a number of significant differences were found. As compared with metrizamide, not only do