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Formation of endogenous pyrogen by bone marrow cells

✍ Scribed by O. M. Efremov; A. V. Sorokin; O. A. Él'kina

Publisher
Springer US
Year
1978
Tongue
English
Weight
166 KB
Volume
85
Category
Article
ISSN
0007-4888

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Fresh marrow cells were obtained from the femora Fischer rats and cultured in a medium containing 15% fetal calf serum (FCS) to leach confluent. After trypsinization, cells were subcultured at a cell density of 100 X 103/35 mm well in the presence of FCS, 10 mM P-glycerophosphate, 82 p g / mL ascorb