Ortho-, meta-, and para-phenylenediamines were polymerized using hydrogen peroxide as an oxidant and horseradish peroxidase as a catalyst in mixed solvents of 1,4-dioxane and water. The yield of the polymers was strongly dependent on solvent composition, and maximum yields were obtained at 15-30% 1,
Formation of Chlorins by Oxidation of Deuteroporphyrin with Horseradish Peroxidase
โ Scribed by Francesc Pont; Nicholas J Jacobs; Franz-Peter Montforts
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- French
- Weight
- 305 KB
- Volume
- 38
- Category
- Article
- ISSN
- 0040-4039
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โฆ Synopsis
Abs#ract: Two novel chlorins 2 and 3 were izolated from the reaction of deuteroporphyrin1 with horseradish ~oxi-and glutathione. On incubation of uroporphyrin I, coproporphyrin III, pmtoporphyrinIX, hematoporphyrinIX and mesoporphyrinIX underthe sameconditionsthe fmmation of chlorinscould not be detected. 0 1997Elsevier ScienceLtd.
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Enzymatic oxidative polymerization of p-alkylphenols using horseradish peroxidase as catalyst has been carried out in two polymerization solvent systems: a mixture of phosphate buffer (pH 7) and 1,4-dioxane, and a reverse micellar solution, yielding powdery polymeric materials. The polymer yield was
Horseradish peroxidase which had been aminated by periodate oxidation and reductive amination was purified by cation-exchange chromatography on S-Sepharose. Instead of the expected single peak of aminated enzyme, two distinct peaks of protein were eluted from the column. Evaluation of the protein in