Formation of C-4 dicarboxylic acids by intact spinach chloroplasts

✍ Scribed by R. Scheibe; E. Beck

• Book ID: 104751002
• Publisher: Springer-Verlag
• Year: 1975
• Tongue: English
• Weight: 312 KB
• Volume: 125
• Category: Article
• ISSN: 0032-0935
• DOI: 10.1007/bf00388873

✦ Synopsis

It is concluded from 14C labelling kinetics as well as from enzyme analysis that formation of malate and aspartate from glycerate-3P via carboxylation of P-enolpyruvate occurs in isolated photosynthesizing spinach (Spinacia oleracea L.) chloroplasts. Carbon incorporation into both dicarboxylic acids was about 1% of the total CO 2 fixation. Both acids are suggested to act as substrates for counter exchange for glutamate and ~.-ketoglutarate.