Formation in agar of fibroblast-like colonies by cells from the mouse pleural cavity and other sources
✍ Scribed by Donald Metcalf
- Publisher
- John Wiley and Sons
- Year
- 1972
- Tongue
- English
- Weight
- 861 KB
- Volume
- 80
- Category
- Article
- ISSN
- 0021-9541
No coin nor oath required. For personal study only.
✦ Synopsis
Colonies of elongated fibroblast-like cells (stellate colonies) developed in agar cultures of mouse pleural cavity cells mixed with whole blood. Cultures of pleural cells alone developed only abortive clusters of round cells. The frequency of colony-forming cells in the pleural cavity was highest i n neonatal mice (200/105 cells) and fell progressively with aging. Stellatc colonyforming cells were not in cell cycle but were radiosensitive. I n adult mice, only occasional colony-forming cells werc detccted in peritoneal cavity, thymic, spleen, lymph node or bone marrow cell populations. Stellate colony formation was not stimulated by the granulopoictic regulator, colony stimulating factor. The active component in whole blood required for stellate colony formation was present i n plasma but not serum or washed red or white cells.