Royal School of Medicine and Dentistry, London, UK. State-of-the-art advances in identity testing through DNA analysis. Covers PCR-based test systems, direct-phase minisequencing, and more. DNLM: DNA Fingerprinting--methods--laboratory manuals.
Forensic DNA Profiling Protocols (Methods in Molecular Biology Vol 98)
โ Scribed by Lincoln, Patrick J.; Thomson, Jim
- Publisher
- Humana Press
- Year
- 1998
- Tongue
- English
- Edition
- 1st
- Category
- Library
No coin nor oath required. For personal study only.
โฆ Synopsis
Leading forensic experts from around the world describe in detail their time-proven methods for identity testing through DNA analysis. Their state-of-the-art collection of easily reproducible methods includes all of the major techniques of DNA analysis currently used in forensic identity testing. The book covers PCR- based test systems, the now widely used STR typing systems (complete with details of both manual and automated detection systems), repeat unit mapping (MVR), direct phase minisequencing, capillary electrophoresis, and direct blotting. Also fully discussed are the recovery of DNA from a wide range of sample types (blood, semen, skeletal remains, and saliva), species testing, sex determination, and mitochondrial DNA testing. This state-of-the-art collection of easily reproducible methods includes all of the major techniques of DNA analysis currently used in forensic identity testing. The methods include the recovery of DNA from a large range of sample types, analysis of DNA as single and multi-locus VNTR probes, PCR amplification of STR and other loci, and mitochondrial sequencing. The expert scientists writing here-many from laboratories around the world-also discuss how to interpret the results in cases of unknown identity and disputed parentage.
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