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Folding of bovine growth hormone is consistent with the molten globule hypothesis

✍ Scribed by Dr. David N. Brems; Dr. Henry A. Havel


Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
250 KB
Volume
5
Category
Article
ISSN
0887-3585

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✦ Synopsis


Previous results from equilibrium and kinetic studies of the folding of bovine growth hormone (bGH) have demonstrated that bGH does not follow a simple two-step folding mechanism. These results are summarized and interpreted according to the "molten globule" model. The molten globule state of bGH is characterized as a folding intermediate which is largely alpha-helical, retains a compact hydrodynamic radius, has packing of the aromatic side chains that is similar to the unfolded state, and possesses a solvent-exposed hydrophobic surface along helix 106-127 that readily leads to association.