Folding and activity of cAMP-dependent protein kinase mutants
β Scribed by Thomas Langer; Sridhar Sreeramulu; Martin Vogtherr; Bettina Elshorst; Marco Betz; Ulrich Schieborr; Krishna Saxena; Harald Schwalbe
- Book ID
- 116465737
- Publisher
- Elsevier Science
- Year
- 2005
- Tongue
- English
- Weight
- 409 KB
- Volume
- 579
- Category
- Article
- ISSN
- 0014-5793
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The signal transduction pathway of cAMP, mediated by the cAMP-dependent protein kinase (PKA), is involved in the regulation of metabolisms, cell growth and differentiation and gene expression. Isolated PKA mutants from Chinese hamster ovary (CHO) cells were used in our laboratory to study the role o
The CAMP-dependent protein kinase (cAK) from Diciyosteliurn discoideurn is an enzyme composed of one catalytic and one regulatory subunit. Upon binding of CAMP, the holoenzyme dissociates to liberate free active catalytic subunits. The cAK is developmentally regulated, ranging from very little activ