Focal adhesion mediated intracellular signaling, Stat3 translocation and osteoblast differentiation: regulation by substratum topography
✍ Scribed by D. W. Hamilton; F. Jamshidi; D. M. Brunette
- Book ID
- 102503351
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 369 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0933-5137
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✦ Synopsis
Abstract
Osseointegration is a necessary process for stabilization of implants that contact bone. Implant substratum topography has been identified as an important modulator of osteoblast differentiation, although the molecular processes involved are poorly understood. The aim of this study was to assess adhesion mediated molecular events induced in rat calvarial osteoblasts by topographies produced using microfabrication techniques. Specifically, we investigated the activation of tyrosine phosphorylation, focal adhesion kinase (FAK), extracellular regulated kinase 1/2 (ERK‐1/2), janus kinase‐1 and 2 (JAK‐1 and 2), and the transcription factor Stat3. Microfabricated topographies stimulated altered focal adhesion (FA) arrangements, which correlated with regions of increased tyrosine phosphorylation. FAK, and ERK 1/2. Inhibition of JAK‐1 using piceatannol attenuated the phosphorylation of FAK and ERK 1/2 on 30μm deep grooves, but not smooth, but inhibited proliferation on all surfaces tested. Inhibition of microtubule nucleation, JAK‐1, JAK‐2 and phospholipase‐C had no effect on nuclear translocation of Stat3 irrespective of topography. We conclude that nuclear translocation of Stat3 is independent of substratum topography, but JAK‐1 is involved in focal adhesion mediated signal transduction. Further understanding of the molecular regulation of osteoblast differentiation by substratum topography will allow the design of more suitable biomaterials for orthopaedic and dental applications.