✦ LIBER ✦

Fmoc-based synthesis of glycolate ester peptides for the assembly of de novo designed multimeric proteins using subtiligase

✍ Scribed by Daniel J Suich; Marcus D Ballinger; James A Wells; William F DeGrado

Publisher: Elsevier Science
Year: 1996
Tongue: French
Weight: 320 KB
Volume: 37
Category: Article
ISSN: 0040-4039
DOI: 10.1016/s0040-4039(96)01444-x

No coin nor oath required. For personal study only.

✦ Synopsis

An automated method utilizing Fmoc-protected amino acids has been developed for the synthesis of glycolate ester peptides as substrates for subtiligase. As a test of this methodology, peptide esters containing o-helical sequences that specify the association into 3-and 4helix bundles were synthesized, and used to explore the suitability of subtiligase as a tool for the generation of covalently linked bundles. The feasibility of creating de nov0 designed, singlechain or cyclic structures from simple, readily-synthesized modules has been demonstrated.

📜 SIMILAR VOLUMES

Total chemical synthesis, characterizati

Total chemical synthesis, characterization, and immunological properties of an MHC class I model using the TASP concept for protein de novo design

✍ G. Tuchscherer; C. Servis; G. Corradin; U. Blum; J. Rivier; M. Mutter 📂 Article 📅 1992 🏛 Cold Spring Harbor Laboratory Press 🌐 English ⚖ 979 KB

## Abstract The design, total chemical synthesis, and immunological properties of a four‐__α__‐helix bundle template‐assembled synthetic protein (TASP) mimicking some of the structural features of the major histocompatibility complex (MHC) class I is described. In a first approach, the native seque