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Fluorometric studies on the binding of gluconolactone, glucose, and glucosides to the subsites of glucoamylase

✍ Scribed by Hiromi, Keitaro; Tanaka, Akiyoshi; Ohnishi, Masatake


Book ID
126489824
Publisher
American Chemical Society
Year
1982
Tongue
English
Weight
727 KB
Volume
21
Category
Article
ISSN
0006-2960

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Binding of isomaltose and maltose to the
✍ Masatake Ohnishi; Takashi Matsumoto; Tatsuya Yamanaka; Keitaro Hiromi πŸ“‚ Article πŸ“… 1990 πŸ› Elsevier Science 🌐 English βš– 676 KB

The binding of maltose, isomaltose, and D-glucono-1,5-lactone to the glucoamylase [E.C.3.2.1.3] from Aspergillus niger was monitored by the fluorescence-intensity change (delta F) based on the tryptophan residues of the enzyme, and the binding parameters (Kd and delta Fmax) were evaluated from the d