Fluorometric Properties of N-Methyltetraphenylporphine and Several Derivatives: Evaluation as Standards for Determination of Chlorophyll Concentrations

Determination of N-Acetyl- and N-Glycoly

Determination of N-Acetyl- and N-Glycolylneuraminic Acids in Gangliosides by Combination of Neuraminidase Hydrolysis and Fluorometric High-Performance Liquid Chromatography Using a GM3 Derivative as an Internal Standard

✍ Toshiyuki Hikita; Keiko Tadano-Aritomi; Naoko Iida-Tanaka; Hidenao Toyoda; Atsus 📂 Article 📅 2000 🏛 Elsevier Science 🌐 English ⚖ 134 KB

A highly sensitive method for quantification of sialic acids in gangliosides was developed. The sialic acids, released by hydrolysis of gangliosides, were converted to fluorescent derivatives with 1,2-diamino-4,5-(methylenedioxy)benzene (DMB) and separated on a reversed-phase C 18 column with an iso