Fluorometric measurement of tin-protoporphyrin in biological samples

✍ Scribed by Creuza S. Simionatto; Karl E. Anderson; Shigeru Sassa; George S. Drummond; Attallah Kappas

• Book ID: 102987138
• Publisher: Elsevier Science
• Year: 1984
• Tongue: English
• Weight: 561 KB
• Volume: 141
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(84)90448-2

✦ Synopsis

Sn-protoporphyrin is a potent competitive inhibitor of heme oxygenase, can suppress neonatal and other forms of hyperbilirubinemia in laboratory animals, and represents a potential new approach to the treatment of neonatal jaundice in humans. In order to study the disposition of Sn-protoporphyrin in vivo we have developed a sensitive fluorometric method for the quantitation of this metalloporphyrin in biological samples. The method is sensitive to concentrations as low as 0.01 nmol/ml, and is specific for Sn-protoporphyrin even in the presence of other porphyrins such as protoporphyrin.

MATERIALS

AND METHODS

Sn4+-protoporphyrin IX and protoporphyrin IX were purchased from Porphyrin

This study was supported by ES01055 Dyson Foundation and the Ogden Corporation.