Fluorometric determination of primary and secondary aliphatic amines by reaction with 9-isothiocyanatoacridine

✍ Scribed by André Deleenheer; J. E. Sinsheimer; J. H. Burckhalter

• Publisher: John Wiley and Sons
• Year: 1973
• Tongue: English
• Weight: 220 KB
• Volume: 62
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600620837

✦ Synopsis

An improved procedure was developed for the analysis of primary and secondary aliphatic amines based upon the fluorescence of a cyclized product derived from isothiourea derivatives of amines and 9-isothiocyanatoacridine. Initial isothiourea formation as well as its cyclization is carried out in toluene, which results in the formation of highly fluorescent 2-alkylamino-l,3-thiazino- [4,5,6-kl]acridine with a minimum of by-products. The fluorescence of these thiazinoacridine compounds is determined either directly in solution after dilution with acidified alcohol or after TLC separation and direct scanning of the TLC plate. For example, amphetamine could be determined with a sensitivity of 0.12 nl./ml. in solution or at a 3-pl. level by the TLC procedure.

Keyphrases 0 Amines, primary and secondary aliphatic-fluorescent analysis with 9-isothiocyanatoacridinc I J Fluorescent analysisprimary and secondary aliphatic amines using 9-isothiocyanatoacridine, TLC fluorometry IJ Amphetamine---fluorescent analysis using 9-isothiocyanatoacridine 0 9-Isothiocyanatoacridine-used