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Fluorometric Determination of EDTA and EGTA Using Terbium–Salicylate Complex

✍ Scribed by Yuan C. Lee

Book ID
102567290
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
59 KB
Volume
293
Category
Article
ISSN
0003-2697

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✦ Synopsis

A method for determining EDTA (ethylenediaminetetraacetic acid) based on a highly fluorescent terbium-EDTA-salicylic acid complex formation was developed. EDTA from as low as a few picomoles to as high as several nanomoles can be determined in a microtiter plate in 10 -20 min. Ethyleneglycol-bis(2aminoethoxy)-tetraacetic acid (EGTA) also can be determined by the same method, but its sensitivity is ca. 14-fold lower. Interestingly, diethylenetriamine tetraacetic acid did not form fluorescent complex with terbium under the same conditions.

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