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Fluorometric detection of implantation sites in rats

✍ Scribed by Hicks, J. J. ;Gil-Recasens, M. E. ;González-zamudio, J.


Publisher
John Wiley and Sons
Year
1980
Tongue
English
Weight
343 KB
Volume
214
Category
Article
ISSN
0022-104X

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✦ Synopsis


Abstract

A new fluorescent technique is proposed for detecting implantation sites in the rat, based on the binding capacity of tetracycline. Both intraluminal (20 μg/kg) and intravenous (8.3 mg/kg) administrations of tetracycline permit the macroscopic visualization (under 366 nm of ultraviolet light) of implantation sites, since they show a lower flurescence (LFS) than the remaining endometrium (FS). Greater binding a tritiated tetracycline was observed in nonimplantation sites (176 to 56 × 10^3^ dpm/mg protein) than in implantation sites (P < 0.05).


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