Fluorometric-colorimetric amino acid analysis of actinomycins using fluorescamine: Mechanism of partial threonine decomposition during acid hydrolysis
✍ Scribed by Arthur M. Felix; John W. Westley; Johannes Meienhofer
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 449 KB
- Volume
- 73
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
A fluorometric-calorimetric fluorescamine analyzer was successfully utilized for the analysis of a series of actinomycin hydrolysates containing N-methylamino acids, proline. and proline derivatives. An explanation is proposed for the low threonine values consistently observed in the analysis of actinomycin hydrolysates and alternate conditions for improved hydrolysis of actinomycins are proposed. ' Fluorescamine; FluramCR): 4-Phenylspiro[furan-2(3H), I'-phthalanl-3,3'-dione. 70 Copyright 0 1976 by Academnc Press. Inc. All rights of reproduction in any form reserved.
* Low values for threonine hydrolysates are well known but only a part of the loss (-5%) can be attributed to deamination during hydrolysis (IO).