Colorimetrical rate assay for urinary di
Colorimetrical rate assay for urinary dipeptidyl peptidase iv (dppiv) activity using a new substrate
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Hiroko Shibuya-Saruta; Masami Sugiyama; Yasushi Kasahara
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Article
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1995
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John Wiley and Sons
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English
⚖ 468 KB
We synthesized a new substrate glycyl-Lproline 3,5-dibromo-4-hydroxyanilide (Gly-Pro-DBAP), for dipeptidyl peptidase IV (DPPIV). Its hydrolysis by DPPlV resulted in the formation of a chromophore, 2,6-dibromophenol-indo-p-xylenol, and its maximal absorption wavelength (600 nm) was longer than that o