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Fluorometric assay for the measurement of viral neuraminidase in influenza vaccines

✍ Scribed by Claude R. Lambré; Sylvie Chauvaux; Yannick Pilatte


Book ID
119150320
Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
198 KB
Volume
7
Category
Article
ISSN
0264-410X

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A fluorometric procedure for quantitating the amount of N-acetylneuraminic acid enzymatically released by the neuraminidase activity from N-acetylneuraminyl-lactose (sialyl-lactose) has been developed. The liberated lactose is hydrolyzed with beta-galactosidase, and the released galactose is oxidize