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Fluorogenic cephalosporin substrates for β-lactamase TEM-1

✍ Scribed by Aleksey Rukavishnikov; Kyle R. Gee; Iain Johnson; Schuyler Corry

Book ID
113412180
Publisher
Elsevier Science
Year
2011
Tongue
English
Weight
593 KB
Volume
419
Category
Article
ISSN
0003-2697

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✍ Ratana Lawung; Bengt Danielsson; Virapong Prachayasittikul; Leif Bülow 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 81 KB

Two ␤-lactamases, penicillinase type I from Bacillus cereus and TEM-1 ␤-lactamase from Haemophilus ducreyi, were immobilized on a Chelating Sepharose Fast Flow column loaded with Ni 2؉ in an active form. Flow-injection analysis of ␤-lactams was performed by using an enzyme column reactor fitted into