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Fluorimetric determination of oxidised and reduced glutathione in cells and tissues by high-performance liquid chromatography following derivatization with dansyl chloride

✍ Scribed by Janet Martin; Ian N.H. White

Publisher
Elsevier Science
Year
1991
Weight
301 KB
Volume
568
Category
Article
ISSN
0378-4347

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✍ Kevin J. Lenton; HΓ©lΓ¨ne Therriault; J.Richard Wagner πŸ“‚ Article πŸ“… 1999 πŸ› Elsevier Science 🌐 English βš– 73 KB

A method is described for the detection of glutathione (GSH) and glutathione disulfide (GSSG) based on a HPLC postcolumn reaction with ortho-phthalaldehyde (OPT) at pH 12 followed by fluorescence detection. Although similar methods have been reported, the high pH of the postcolumn reaction adds cons