Fluorimetric determination of danthron in pharmaceutical tablets and in urine

✍ Scribed by Blair E Miller; Neil D Danielson

• Book ID: 104100266
• Publisher: Elsevier Science
• Year: 1987
• Tongue: English
• Weight: 444 KB
• Volume: 192
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/s0003-2670(00)85714-1

✦ Synopsis

A simple, rapid determination is reported for danthron (1,8-dihydroxyanthraquinone) in pharmaceutical tablets. In a flow-injection system, danthron is reduced by sodium dithionite in l/l methanol/borate buffer to give a fluorescent complex. Linearity ranges from 30 E.cg ml-' to below 0.1 Mg ml-' . In urine samples, danthron is separated from other fluorescing species by reversed-phase high-performance liquid chromatography before its reduction by dithionite in a post-column reactor. Urine preparation requires no extraction. Spiked urine samples were studied in the working range of 0.02-2.0 pg ml-' danthron.