## Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a βFull Textβ option. The original article is trackable v
Fluorescent properties of model chromophores of tyrosine-66 substituted mutants of Aequorea green fluorescent protein (GEP)
β Scribed by Satoshi Kojima; Hiroko Ohkawa; Takashi Hirano; Shojiro Maki; Haruki Niwa; Mamoru Ohashi; Satoshi Inouye; Frederick I. Tsuji
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- French
- Weight
- 245 KB
- Volume
- 39
- Category
- Article
- ISSN
- 0040-4039
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β¦ Synopsis
In an ethanol-glass marix at 77 K, model compounds of the chromophores of recombinant GFP-Y66F, GFP-Y66W, and GFP-Y66H exhibited fluorescence properties close to those of the corresponding mutant proteins.
π SIMILAR VOLUMES
Aequoreu green fluorescent protein (GFP) has been expressed in a variety of cell lines and host organisms. A recent report (Heim et d.: Proc Natl mented that a GFP mutant with a single amino acid substitution (tyrosine 66 to histidine; Y66H-GFP) elicits altered spectral properties. Whereas wildtype
## Abstract Molecular dynamics (MD) simulations were carried out to study the conformational rearrangement induced by deprotonation of the fluorescent chromophore in GFP, as well as the associated changes in the hydrogenβbonding network. For both the structures with either a neutral or an anionic c
The origin of the low steady-state fluorescence quantum yield of some blue-emitting variants of the green fluorescent protein (GFP) is investigated in single-site mutants in which the tyrosine residue at position 66 has been replaced by phenylalanine or by histidine. Time-resolved fluorescence measu
## Abstract Green fluorescent proteins (GFPs) are widely used as tools in biochemistry, cell biology, and molecular genetics due to their unusual optical spectroscopic characteristics. The spectrophotometric and fluorescence properties of GFPs are controlled by the protonation states and possibly _