𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Fluorescent properties of model chromophores of tyrosine-66 substituted mutants of Aequorea green fluorescent protein (GEP)

✍ Scribed by Satoshi Kojima; Hiroko Ohkawa; Takashi Hirano; Shojiro Maki; Haruki Niwa; Mamoru Ohashi; Satoshi Inouye; Frederick I. Tsuji


Publisher
Elsevier Science
Year
1998
Tongue
French
Weight
245 KB
Volume
39
Category
Article
ISSN
0040-4039

No coin nor oath required. For personal study only.

✦ Synopsis


In an ethanol-glass marix at 77 K, model compounds of the chromophores of recombinant GFP-Y66F, GFP-Y66W, and GFP-Y66H exhibited fluorescence properties close to those of the corresponding mutant proteins.


πŸ“œ SIMILAR VOLUMES


ChemInform Abstract: Fluorescent Propert
✍ S. KOJIMA; H. OHKAWA; T. HIRANO; S. MAKI; H. NIWA; M. OHASHI; S. INOUYE; F. I. T πŸ“‚ Article πŸ“… 2010 πŸ› John Wiley and Sons βš– 27 KB πŸ‘ 2 views

## Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a β€œFull Text” option. The original article is trackable v

Aequorea green fluorescent protein: Simu
✍ J. Dezz Ropp; Christopher J. Donahue; David Wolfgang-Kimball; Jeffrey J. Hooley; πŸ“‚ Article πŸ“… 1996 πŸ› John Wiley and Sons 🌐 English βš– 552 KB

Aequoreu green fluorescent protein (GFP) has been expressed in a variety of cell lines and host organisms. A recent report (Heim et d.: Proc Natl mented that a GFP mutant with a single amino acid substitution (tyrosine 66 to histidine; Y66H-GFP) elicits altered spectral properties. Whereas wildtype

Molecular modeling of green fluorescent
✍ Soumya S. Patnaik; Steven Trohalaki; Ruth Pachter πŸ“‚ Article πŸ“… 2004 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 245 KB

## Abstract Molecular dynamics (MD) simulations were carried out to study the conformational rearrangement induced by deprotonation of the fluorescent chromophore in GFP, as well as the associated changes in the hydrogen‐bonding network. For both the structures with either a neutral or an anionic c

Picosecond Time-Resolved Fluorescence fr
✍ Andreas D. Kummer; Jens Wiehler; Tanja A. SchΓΌttrigkeit; Bryan W. Berger; Boris πŸ“‚ Article πŸ“… 2002 πŸ› John Wiley and Sons 🌐 English βš– 120 KB

The origin of the low steady-state fluorescence quantum yield of some blue-emitting variants of the green fluorescent protein (GFP) is investigated in single-site mutants in which the tyrosine residue at position 66 has been replaced by phenylalanine or by histidine. Time-resolved fluorescence measu

Electronic excitations of green fluoresc
✍ Daiqian Xie; Jun Zeng πŸ“‚ Article πŸ“… 2005 πŸ› John Wiley and Sons 🌐 English βš– 183 KB

## Abstract Green fluorescent proteins (GFPs) are widely used as tools in biochemistry, cell biology, and molecular genetics due to their unusual optical spectroscopic characteristics. The spectrophotometric and fluorescence properties of GFPs are controlled by the protonation states and possibly _