Fluorescent Measurement of TNS Binding to Calmodulin: A Biochemistry Laboratory Experiment
β Scribed by Buccigross, Jeanne M.; Bedell, Cynthia M.; Suding-Moster, Heather L.
- Book ID
- 120976659
- Publisher
- American Chemical Society
- Year
- 1996
- Tongue
- English
- Weight
- 633 KB
- Volume
- 73
- Category
- Article
- ISSN
- 0021-9584
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
The interaction of cyclosporin \(A\) and cyclosporin derivatives with cyclophilins \(A, B\), and \(C\) has been investigated by means of fluorescence measurement techniques. Since Trp-121 of cyclophilin \(A\) is in close contact with bound cyclosporins and changes its fluorescence emission upon bind
A sensitive fluorescence resonance energy transfer method was developed for the direct measurement of the dissociation constants of stromelysin inhibitors. The method is applied to the thiadiazole class of stromelysin inhibitors and it takes advantage of the fact that, upon binding to the active sit