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Fluorescent colloidal gold: A cytochemical marker for fluorescent and electron microscopy

✍ Scribed by M. Horisberger; Monique Vonlanthen

Publisher
Springer
Year
1979
Tongue
English
Weight
714 KB
Volume
64
Category
Article
ISSN
1432-119X

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✦ Synopsis

The gold method was further developed for fluorescent microscopy. Gold granules (12 nm in size) were labelled with rhodamine conjugates of Concanavalin A and avidin. The fluorescent markers were used to mark cell wall mannan on the yeast Saccharomyces cerevisiae either by the one-step, or by the two-step method via a biotinyl derivative of ConA. By fluorescence or transmission electron microscopy, the two-step method was found to achieve a higher density of marking.

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Immunoprobes which incorporate both a fluorescent label and a 1.4 nm gold cluster compound were prepared by covalent conjugation to FabЈ antibody fragments of the Nanogold cluster label followed by a fluorescent moiety. These new immunoconjugates allow the collection of two complementary sets of dat