Fluorescence study of guanidine hydrochloride denaturation of thymidylate synthetase
โ Scribed by Lloyd A. May; H.C. Cheung; J.L. Aull; J.D. Glickson
- Book ID
- 118310944
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 294 KB
- Volume
- 73
- Category
- Article
- ISSN
- 0006-291X
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Several mutant forms of staphylococcal nuclease with one or two defined amino acid substitutions have been purified, and the effects of the altered amino acid sequence on the stability of the folded conformation have been analyzed by guanidine hydrochloride denaturation. Two nuc-mutations, which gre
Two fluorescent derivatives of 2'-deoxy-5-fluorouridine 5'-p-aminophenyl phosphate were prepared by treatment of this compound with fluorescein isothiocyanate in dimethyl sulfoxide or 5-(dimethylamino)naphthalene sulfonyl chloride in pyridine. The products of the reactions were isolated by diethylam