Fluorescence studies on interaction between phospho-LHC II and subchloroplast Photosystem 1 preparations

✍ Scribed by Svetlana M. Kochubey; Victor V. Shevchenko; Olga I. Volovik

• Publisher: Springer
• Year: 1993
• Tongue: English
• Weight: 454 KB
• Volume: 38
• Category: Article
• ISSN: 0166-8595
• DOI: 10.1007/bf00146414

✦ Synopsis

Chloroplast proteins were phosphorylated under two test conditions: 'white light' irradiance alone and 'white light' irradiance with the addition of glucose and glucose oxidase, used to produce an anaerobic medium. The interaction of phospho-LHC II with Photosystem 1 (PS 1) was studied for two types of PSI preparation. Changes in the chlorophyll a/b ratio and the ratio of 650 and 680 nm band intensities (E650/E680) in fluorescence excitation spectra were used in calculating the phospho-LHC II portion which became associated with PS 1. It is shown that the associated portion of phospho-LHC II varies for each of the PS 1 preparations and phosphorylation procedures. Possible conclusions as regards the transfer of various sets of LHC II subpopulations under different phosphorylation procedures and the differences of interaction with PS 1 are discussed.