Fluorescence spectroscopy of epithelial tissue throughout the dysplasia-carcinoma sequence in an animal model: Spectroscopic changes precede morphologic changes
✍ Scribed by Lezlee Coghlan; Urs Utzinger; Rebecca Richards-Kortum; Carrie Brookner; Andres Zuluaga; Irma Gimenez-Conti; Michele Follen
- Book ID
- 102467819
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 194 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0196-8092
- DOI
- 10.1002/lsm.1078
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Background and Objective
The hamster cheek pouch carcinogenesis model, using chronic treatments of dimethylbenz[α]anthracene (DMBA) was used as a model system to investigate changes in epithelial tissue autofluorescence throughout the dysplasia‐carcinoma sequence.
Study Design/Materials and Methods
Fluorescence emission spectra were measured weekly from 42 DMBA‐treated animals and 20 control animals at 337, 380, and 460 nm excitation. A subset of data in which histopathology was available was used to develop diagnostic algorithms to separate neoplastic and non‐neoplastic tissue. The change in fluorescence intensity over time was examined in all samples at excitation‐emission wavelength pairs identified as diagnostically useful.
Results
Algorithms based on autofluorescence can separate neoplastic and non‐neoplastic tissue with 95% sensitivity and 93% specificity. Greatest contributions to diagnostic algorithms are obtained at 380 nm excitation, and 430, 470, and 600 nm emission. Changes in fluorescence intensity are apparent as early as 3 weeks after initial treatment with DMBA, whereas morphologic changes associated with dysplasia occur on average at 7.5–12.5 weeks after initial treatment.
Conclusions
Fluorescence spectroscopy provides a potential tool to identify biochemical changes associated with dysplasia and hyperplasia, which precede morphologic changes observed in histologically stained sections. Lasers Surg. Med. 29:1–10, 2001. © 2001 Wiley‐Liss, Inc.