Fluorescence spectroscopy as a tool for monitoring solubility and aggregation behavior of β-lactoglobulin after heat treatment
✍ Scribed by Rand Elshereef; Hector Budman; Christine Moresoli; Raymond L. Legge
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 559 KB
- Volume
- 95
- Category
- Article
- ISSN
- 0006-3592
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✦ Synopsis
Abstract
Denaturation and aggregation of whey proteins are of interest to the food and pharmaceutical industry due to the importance of final structure in functionality, impact on food texture, and the chemical stability of the final product. In this study, we demonstrate the potential of fluorescence spectrometry combined with multivariate chemometric methods for quantifying solubility and aggregation behavior of β‐lactoglobulin (β‐LG); a major whey protein and a frequent food ingredient. Heat‐induced aggregation of β‐LG was studied under different conditions including pH, temperature and heating durations. Results showed very good agreement between the fluorescence‐based predictions and measurements obtained by HPLC and gravimetric analysis regardless of the conditions. Standard normal variate (SNV), a signal preprocessing and filtering tool, was found to enhance the predictive accuracy and robustness of the fluorescence‐based model. © 2006 Wiley Periodicals, Inc.