Fluorescence resonance energy transfer between blue-emitting and red-shifted excitation derivatives of the green fluorescent protein
β Scribed by Robi D. Mitra; Christopher M. Silva; Douglas C. Youvan
- Book ID
- 116133313
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 384 KB
- Volume
- 173
- Category
- Article
- ISSN
- 0378-1119
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Background: Fluorescence resonance energy transfer (FRET) is a powerful technique for measuring molecular interactions at Γ ngstrom distances. We present a new method for FRET that utilizes the unique spectral properties of variants of the green fluorescent protein (GFP) for large-scale analysis by f
Enhanced blue fluorescent protein (EBFP) and enhanced green fluorescent protein (EGFP) mutants of GFP in close proximity to one another can act as a fluorescence resonance energy transfer (FRET) pair. Unstructured amino acid linkers of varying length were inserted between EBFP and EGFP, revealing th