Fluorescence resonance energy transfer analysis of proapoptotic CD95–EGF receptor interactions in Huh7 cells

Fluorescence resonance energy transfer a

Fluorescence resonance energy transfer analysis of cell surface receptor interactions and signaling using spectral variants of the green fluorescent protein

✍ Francis Ka-Ming Chan; Richard M. Siegel; David Zacharias; Ruth Swofford; Kevin L 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 322 KB 👁 2 views

Background: Fluorescence resonance energy transfer (FRET) is a powerful technique for measuring molecular interactions at Ångstrom distances. We present a new method for FRET that utilizes the unique spectral properties of variants of the green fluorescent protein (GFP) for large-scale analysis by f