𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Fluorescence recovery after photobleaching measurements of polymers in a surface forces apparatus

✍ Scribed by Janet S. S. Wong; Liang Hong; Sung Chul Bae; Steve Granick

Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
595 KB
Volume
48
Category
Article
ISSN
0887-6266

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The surface forces apparatus has been combined with fluorescence recovery after photobleaching to measure translational diffusion of polymer confined between mica sheets. This article presents findings using polydimethylsiloxane with number‐average molecular weight M~n~ = 2200 g mol^−1^, the chains end‐labeled with soluble fluorescent dye. Melts with thickness 10 nm display a translational diffusion coefficient (D) with a bulk component and a slower component assigned to surface diffusion. Reduction of thickness to 1.8 nm causes mobility to split into two populations—an immobile fraction (immobile on the time scale of 30–60 min) and a mobile fraction who's D slow only weakly with diminishing film thickness. However, when load causes the confining mica sheets to flatten, D of the mobile fraction drops by up to an additional order of magnitude, depending on the local pressure that squeezes on the polymer. © 2010 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys, 2010

📜 SIMILAR VOLUMES

Force meets chemistry: Analysis of mecha
Force meets chemistry: Analysis of mechanochemical conversion in focal adhesions using fluorescence recovery after photobleaching
✍ Tanmay P. Lele; Charles K. Thodeti; Donald E. Ingber 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 104 KB

## Abstract Mechanotransduction—the process by which mechanical forces are converted into changes of intracellular biochemistry—is critical for normal cell and tissue function. Integrins facilitate mechanochemical conversion by transferring physical forces from the extracellular matrix, across the

Measurement of the lateral mobility of c
Measurement of the lateral mobility of cell surface components in single living cells by fluorescence recovery after photobleaching
✍ Jacobson, K. ;Derzko, Z. ;Wu, E-S. ;Hou, Y. ;Poste, G. 📂 Article 📅 1976 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 707 KB

## Abstract The use of fluorescence recovery after photobleaching (FRAP) techniques to monitor the lateral mobility of plant lectin‐receptor complexes on the surface of single, living mammalian cells is described in detail. FRAP measurements indicate that over 75% of the wheat germ agglutinin recep

Application of fluorescence recovery aft
Application of fluorescence recovery after photobleaching to diffusion of a polyelectrolyte in a multilayer film
✍ Catherine Picart; Jérôme Mutterer; Youri Arntz; Jean-Claude Voegel; Pierre Schaa 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 828 KB

## Abstract The diffusion coefficient, __D__, and the proportion of mobile molecules, __p__, is measured for a fluorescently labeled polyelectrolyte in a multilayer film using fluorescence recovery after photobleaching (FRAP). The film was composed of poly(L‐lysine) (PLL) and hyaluronan (HA). The l

Fluorescence recovery after photobleachi
Fluorescence recovery after photobleaching and photoconversion in multiple arbitrary regions of interest using a programmable array microscope
✍ Guy M. Hagen; Wouter Caarls; Keith A. Lidke; Anthony H.B. De Vries; Cornelia Fri 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 480 KB

## Abstract Photomanipulation (photobleaching, photoactivation, or photoconversion) is an essential tool in fluorescence microscopy. Fluorescence recovery after photobleaching (FRAP) is commonly used for the determination of lateral diffusion constants of membrane proteins, and can be conveniently