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Fluorescence quenching studies of Trp repressor using single-tryptophan mutants

✍ Scribed by Zofia Blicharska; Zygmunt Wasylewski

Book ID
105390113
Publisher
Springer
Year
1995
Tongue
English
Weight
697 KB
Volume
14
Category
Article
ISSN
1573-4943

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## Abstract In order to determine environments around four tryptophan residues, located in the N‐terminus, in the kinase and in the phosphatase domains of rat testis Fru 6‐P,2‐kinase:Fru 2,6‐bisphosphatase, mutant enzymes containing a single tryptophan were constructed by site‐directed mutagenesis.