๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Fluorescence quenching of a series of membrane probes measured in living cells by flow cytometry

โœ Scribed by Dr. James M. Collins; W. McLean Grogan

Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
478 KB
Volume
12
Category
Article
ISSN
0196-4763

No coin nor oath required. For personal study only.

โœฆ Synopsis

The transverse location normal to the bilayer surface of a series of n-(9-anthroyloxy) fatty acid probes, where n = 2, 3, 6, 7, 9, 12, and 16, was determined by fluorescence quenching measurements with a flow cytometer. We show that the anthroyloxy moieties of the probes locate at a graded series of depths in the outer leaflet of the plasma membrane of living HeLa cells, in a manner similar to that previously observed for model membrane systems, and mitochondria. For different n, the efficiency of quenching with an aqueous phase quencher, Cu+2, was 2 greater than or equal to 3 greater than 6 greater than or equal to 7 greater than 9 greater than 12 greater than 16. Therefore, flow cytometry permits use of these probes for measurements of dynamic parameters related to membrane fluidity at different depths in the plasma membranes of living cells.

๐Ÿ“œ SIMILAR VOLUMES

Fluorescence spectra of cells stained wi
Fluorescence spectra of cells stained with a DNA-specific dye, measured by flow cytometry
โœ Harald B. Steen; Trond Stokke ๐Ÿ“‚ Article ๐Ÿ“… 1986 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 270 KB

Fluorescence spectra of ethanol-fixed rat thymocytes stained with the DNA-specific dye Hoechst 33258 have been measured in an arc lamp-based flow cytorneter including a grating monochromator in front of the fluorescence detector. Spectral resolution was 5-10 nm. Increasing dye concentration was foun

Use of a membrane-localized green fluore
Use of a membrane-localized green fluorescent protein allows simultaneous identification of transfected cells and cell cycle analysis by flow cytometry
โœ Robert F. Kalejta; Thomas Shenk; Andrew J. Beavis ๐Ÿ“‚ Article ๐Ÿ“… 1997 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 110 KB ๐Ÿ‘ 2 views

The simultaneous detection of the green fluorescent protein (GFP) and DNA content using propidium iodide (PI) by flow cytometry is made difficult because of the unique nature of these 2 fluorogenic reagents. For PI to enter cells efficiently and to stain DNA quantitatively, the cells must first be p

Comparative uptake of adriamycin and dau
Comparative uptake of adriamycin and daunorubicin in sensitive and resistant friend leukemia cells measured by flow cytometry
โœ H. Tapiero; A. Fourcade; P. Vaigot; J. J. Farhi ๐Ÿ“‚ Article ๐Ÿ“… 2005 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 473 KB

## Abstract Based on the fluorescence properties of adriamycin (ADM) and daunorubicin (DNR), uptake in sensitive and resistant Friend leukemia cells (FLC) was studied with the aid of the fluorescence activated cell sorter (FACS II). A quantitative cell by cell fluorescence intensity analysis showed

Proportion of S-phase tumor cells measur
Proportion of S-phase tumor cells measured by flow cytometry is an independent prognostic factor in meningioma tumors
โœ A. Maรญllo; P. Dรญaz; A. Blanco; A. Lรณpez; J. Ciudad; J. Hernรกndez; F. Morales; J. ๐Ÿ“‚ Article ๐Ÿ“… 1999 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 108 KB ๐Ÿ‘ 1 views

Meningiomas are tumors in arachnoid cells which represent up to one fifth of all intracranial tumors and up to a quarter of spinal neoplasias. Although meningiomas have classically been considered to be benign tumors, it has also been well-established that they show a heterogeneous clinical outcome.