Fluorescence polarization of trypsin digested photosystem II membranes

✍ Scribed by Ranjana Paliwal; G. S. Singhal

• Book ID: 104615205
• Publisher: Springer
• Year: 1987
• Tongue: English
• Weight: 347 KB
• Volume: 12
• Category: Article
• ISSN: 0166-8595
• DOI: 10.1007/bf00019153

✦ Synopsis

Fluorescence polarization of photosystem II particles treated with trypsin and incubated with high salt-medium (2M NaCl) was investigated. The presence of atrazine and TMPD in normal and salt-washed particles induced a decrease in the polarization ratios. Similar results were obtained at low concentrations of trypsin. On the basis of our observations we suggest that the presence of these perturbing agents causes a reorganisation of the membrane components and alters pigment-pigment and pigment-protein interactions. The results of fluorescence polarization demonstrate trypsin entry into the membrane after the digestion of the peripheral proteins.