Fluorescence polarization characteristics of the complexes between ethidium bromide and rRNA, tRNA, and DNA
β Scribed by V.W.F. Burns
- Book ID
- 115700896
- Publisher
- Elsevier Science
- Year
- 1971
- Tongue
- English
- Weight
- 594 KB
- Volume
- 145
- Category
- Article
- ISSN
- 0003-9861
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## Abstract Ethidium bromide inhibits the formation of ultravioletβinduced pyrimidine dimers in DNA. The efficiency of dimer inhibition increases with increasing energy of the exciting photons. The efficiency of energy transfer from the DNA singlet to the dye singlet, as monitored by sensitized flu
Until now the fluorescence assay with ethidium bromide has only been used on pure DNA. This assay depends on the difference in fluorescence between single-and double-stranded D N A (dsDNA). Cross-links in D N A are measured by the return of fluorescence of dsDNA after heat denaturation at PH 12. Und