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Fluorescence Polarization: Analysis of Carbohydrate–Protein Interaction

✍ Scribed by Kazuaki Kakehi; Yasuo Oda; Mitsuhiro Kinoshita

Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
158 KB
Volume
297
Category
Article
ISSN
0003-2697

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✦ Synopsis

Fluorescence polarization has been widely used for the studies on the molecular motion in solution and has been applied to immunoassays for proteins, therapeutic drug monitoring in clinical pharmacy, and assays for environmentally toxic compounds. Because fluorescence polarization is most readily applicable to the kinetic analysis of the binding reaction between a substance having small molecular mass and a receptor molecule, this method is easily applied to the analysis of carbohydrate-lectin binding. In this tutorial Thematic Review, we briefly introduce the principles of fluorescence polarization and some applications of fluorescence polarization technique to glycobiology.

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