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Fluorescence of proteins induced by two-photo absorption

✍ Scribed by Ying-Wu Xu; Jing-Ru Zhang; Yu-Mei Deng; Ling-Kai Hui; Shou-Ping Jiang; Shao-Hui Lian

Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
330 KB
Volume
1
Category
Article
ISSN
1011-1344

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✦ Synopsis

At room temperature, the UV fluorescence of trypsin, thyroglobulin, hemoglobin and albumin are observed; these molecules undergo two-photon absorption when they are excited with the radiation of a frequency-duplicated Q-switched Nd:YAG laser. It is shown that the fluorescence of these proteins comes from the tryptophan residues and that it has a red shift of about 20 nm relative to the one-photon fluorescence. We suggest that the weak emission from tyrosine arises from the forbidden two-photon transition. The influence of concentration on the fluorescence of hemoglobin and tryptophan is discussed. The two-photon absorption cross-sections are estimated.

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