Fluorescence Microscopy. Super-Resolution and Other Novel Techniques

✍ Scribed by Anda Cornea and P. Michael Conn (Eds.)

• Publisher: Academic Press
• Year: 2014
• Tongue: English
• Leaves: 245
• Edition: 1
• Category: Library

✦ Synopsis

Fluorescence Microscopy: Super-Resolution and other Novel Techniques delivers a comprehensive review of current advances in fluorescence microscopy methods as applied to biological and biomedical science. With contributions selected for clarity, utility, and reproducibility, the work provides practical tools for investigating these ground-breaking developments. Emphasizing super-resolution techniques, light sheet microscopy, sample preparation, new labels, and analysis techniques, this work keeps pace with the innovative technical advances that are increasingly vital to biological and biomedical researchers.

With its extensive graphics, inter-method comparisons, and tricks and approaches not revealed in primary publications, Fluorescence Microscopy encourages readers to both understand these methods, and to adapt them to other systems. It also offers instruction on the best visualization to derive quantitative information about cell biological structure and function, delivering crucial guidance on best practices in related laboratory research.

• Presents a timely and comprehensive review of novel techniques in fluorescence imaging as applied to biological and biomedical research
• Offers insight into common challenges in implementing techniques, as well as effective solutions
• Provides video accompaniment for certain techniques on a companion website for a multi-dimensional perspective: http://booksite.elsevier.com/9780124095137

✦ Table of Contents

Content:
Front Matter, Page iii
Copyright, Page iv
Preface, Page vii
List of Contributors, Page ix, Daniel Axelrod, Melanie Bokstad, Martin J. Booth, Julian Borejdo, Alessandra Cambi, Julien Colombelli, Ali Ertürk, Eugenio F. Fornasiero, Sung-Sik Han, Samie R. Jaffrey, Min-Kyo Jung, Sandra de Keijzer, Jun-Sung Kim, Lynlee L. Lin, Er Liu, Corinne Lorenzo, Ohad Medalia, Marjolein B.M. Meddens, John P. Nolan, Felipe Opazo, Chan-Gi Pack, et al.
Chapter 1 - Evanescent Excitation and Emission, Pages 1-14, Daniel Axelrod
Chapter 2 - Adaptive Optics for Fluorescence Microscopy, Pages 15-33, Martin J. Booth, Brian R. Patton
Chapter 3 - Rapid Measurements of Orientation and Rotation in Ex Vivo Muscle Made Possible by Studying Small Number of Cross-Bridges, Pages 35-48, Julian Borejdo
Chapter 4 - High Spatiotemporal Bioimaging Techniques to Study the Plasma Membrane Nanoscale Organization, Pages 49-63, Marjolein B.M. Meddens, Sandra de Keijzer, Alessandra Cambi
Chapter 5 - High-Resolution 3D Imaging of Intact Transparent Organs by 3DISCO, Pages 65-81, Ali Ertürk
Chapter 6 - Using RNA Mimics of GFP to Image RNA Dynamics in Mammalian Cells, Pages 83-91, Rita L. Strack, Samie R. Jaffrey
Chapter 7 - Surface Enhanced Raman Scattering (SERS) Image Cytometry for High-Content Screening, Pages 93-108, Er Liu, John P. Nolan
Chapter 8 - Light Sheet Fluorescence Microscopy Applications for Multicellular Systems, Pages 109-120, Julien Colombelli, Corinne Lorenzo
Chapter 9 - Correlative Light Electron Microscopy as a Navigating Tool for Cryo-Electron Tomography Analysis, Pages 121-131, Melanie Bokstad, Ohad Medalia
Chapter 10 - Cellular and Molecular Applications of Super-resolution Microscopy, Pages 133-152, Eugenio F. Fornasiero, Felipe Opazo
Chapter 11 - Use of Engineered Nanoparticle-Based Fluorescence Methods for Live-Cell Phenomena, Pages 153-169, Chan-Gi Pack, Min-Kyo Jung, Mi-Roung Song, Jun-Sung Kim, Sung-Sik Han, Yasushi Sako
Chapter 12 - High-Resolution Estimation of Multiple Cell Populations in Tissue Using Confocal Stereology, Pages 171-184, Daniel A. Peterson
Chapter 13 - Multiphoton Microscopy Applications in Biology, Pages 185-197, Miko Yamada, Lynlee L. Lin, Tarl W. Prow
Chapter 14 - Super-resolution Microscopy: A Comparison of Commercially Available Options, Pages 199-212, Jennifer A. Thorley, Jeremy Pike, Joshua Z. Rappoport
Chapter 15 - Structured Illumination Microscopy, Pages 213-225, Lin Shao, E. Hesper Rego
Chapter 16 - The Role of Image Analysis Algorithms in Super-resolution Localization Microscopy, Pages 227-242, Alex Small, Shane Stahlheber
Index, Pages 243-249